Dynamic Visualization of Cellular Signaling
June 21st, 2009 Posted by: admin
Our understanding of cellular signaling is critically dependent on our ability to visualize and quantify specific signaling
events with high spatial and temporal resolution in the cellular context. Over the past decade or so, biosensors based on
fluorescent proteins and fluorescence resonance energy transfer (FRET) have emerged as one major class of fluorescent probes
that are capable of tracking a variety of cellular signaling events, such as second messenger dynamics and enzyme activation/activity,
in time and space. Here we review recent advances in the development of such biosensors and some biological insights revealed
by these biosensors in living cells, tissue, and organisms.
events with high spatial and temporal resolution in the cellular context. Over the past decade or so, biosensors based on
fluorescent proteins and fluorescence resonance energy transfer (FRET) have emerged as one major class of fluorescent probes
that are capable of tracking a variety of cellular signaling events, such as second messenger dynamics and enzyme activation/activity,
in time and space. Here we review recent advances in the development of such biosensors and some biological insights revealed
by these biosensors in living cells, tissue, and organisms.
- Content Type Book Chapter
- DOI 10.1007/10_2008_48
- Authors
- Qiang Ni
- Jin Zhang, The Johns Hopkins University School of Medicine Department of Pharmacology and Molecular Sciences Baltimore MD 21205 USA
- Book Series Advances in Biochemical Engineering/Biotechnology
- Online ISSN 1616-8542
- Print ISSN 0724-6145
